The Contribution of Cytosolic Group IVA and Calcium-Independent Group VIA Phospholipase A2s to Adrenic Acid Mobilization in Murine Macrophages

Coordinate Regulation of Toll-like Receptor-mediated Arachidonic Acid Mobilization in Macrophages by Group IVA and Group V Phospholipase A2s

Macrophages can be activated through Toll-like receptors (TLR) for a variety of innate immune responses. In contrast with the wealth of data existing on TLRdependent gene expression and resultant cytokine production, very little is known on the mechanisms governing TLR-mediated arachidonic acid (AA) mobilization and subsequent eicosanoid production. We have previously reported the involvement o...

Cytosolic group IVA and calcium-independent group VIA phospholipase A2s act on distinct phospholipid pools in zymosan-stimulated mouse peritoneal macrophages.

Phospholipase A2s generate lipid mediators that constitute an important component of the integrated response of macrophages to stimuli of the innate immune response. Because these cells contain multiple phospholipase A2 forms, the challenge is to elucidate the roles that each of these forms plays in regulating normal cellular processes and in disease pathogenesis. A major issue is to precisely ...

Phospholipase A Macrophages by Group IVA and Group V Arachidonic Acid Mobilization in Coordinate Regulation of TLR-Mediated

Localization and functional interrelationships among cytosolic Group IV, secreted Group V, and Ca2+-independent Group VI phospholipase A2s in P388D1 macrophages using GFP/RFP constructs.

P388D(1) cells exposed to bacterial lipopolysaccharide (LPS) mobilize arachidonic acid (AA) for prostaglandin synthesis in two temporally distinct pathways. The "immediate pathway" is triggered within minutes by receptor agonists such as platelet-activating factor (PAF) but only if the cells have previously been primed with LPS for 1 h. The "delayed pathway" occurs in response to LPS alone over...

Cytosolic Group IVA and Calcium-independent Group VIA Phospholipases A2 Act on Distinct Phospholipid Pools in Zymosan- Stimulated Mouse Peritoneal Macrophages